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Flow Cytometry is a technique for counting, examining and sorting microscopic particles suspended in a stream of fluid. It allows simultaneous multiparametric analysis of the physical or chemical characteristics of single cells flowing through an optical or electronic detection apparatus. Modern flow cytometers are able to analyse several thousand particles every second, in 'real time' and can actively separate and isolate particles having specified properties. A flow cytometer has 5 main components:a flow cell, a light source - commonly used are lamps, high power water-cooled lasers, low power air-cooled lasers, a detector and analogue to digital conversion system , an amplification system - linear or logarithmic and a computer for analysis of the signals.
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